| Degree and area of specialization:
Bachelor's Degree in molecular biology/ biological sciences is required. Minimum number of years and type of relevant work experience: Minimum work experience of at least 1-2 years of previous laboratory experience and experience with experimental cerebral ischemia surgery in rats/mice is required. Principal duties:
The research specialist will perform research related to understanding the role of the innate immune system in repair and recovery following focal cerebral ischemia. 50% experimental ischemia surgeries in rats/mice - Performing Middle Cerebral Artery Occlusion surgeries in rats and mice
- Using Laser Doppler to check cerebral blood flow during above surgeries
- Post-op care of MCAO animals
- Neurobehavioral assessment of animals post-op
- Dissection, collection and preparation of brains after sacrifice - Sacrifice and perfusion of above animals after specific time points
15% Molecular biology, Histology and Microscopy, tissue culture techniques - RT-PCR, qPCR, RNA sequencing and Western blots
- Analysis of gene expression in rodent and human brain tissues
- Microscopy: bright field and fluorescent techniques, 3-D imaging
- Histology: Preparation of rodent brains for cryosectioning and immunofluorescence histochemistry
- Maintain cell lines in culture.
10% General lab duties: - Purchasing and managing inventory of laboratory supplies and general lab maintenance.
- Performing required recordkeeping such as hazardous waste disposal
- Training and working with students and undergraduate helpers.
- Resolve unexpected/unplanned issues as they arise.
15% Assist and teach students lab techniques and procedures and help others with their experiments
10% Semi-independent research project responsibilities POSITION VACANCY LISTING University of Wisconsin-Madison Additional Information:
Good written and oral communication skills and ability to perform literature searches.
A 12 month evaluation period will be required. Position Summary:
The candidate will perform experimental focal ischemia surgery in rodents and perform other biological/molecular biology techniques such as Western blotting, tissue culture, PCR, etc., as well as lab managerial duties. The School of Medicine and Public Health has a deep and profound commitment to diversity both as an end in itself but also as a valuable means for eliminating health disparities. As such, we strongly encourage applications from candidates who foster and promote the values of diversity and inclusion.
A period of evaluation will be required. | 
